Compact Square-Wave Pulse Electroporator with Controlled Electroporation Efficiency and Cell Viability
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چکیده
منابع مشابه
Highly efficient oligonucleotide transfer into intact yeast cells using square-wave pulse electroporation.
Here, we present a rapid and reproducible procedure based on square-wave pulse electroporation that allows efficient penetration of synthetic oligonucleotides into intact yeast cells. This procedure was successfully used to modify the yeast genome with small amounts of oligonucleotide.
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Extraction of intracellular proteins from cells is often an important first step for conducting molecular biology and proteomics studies. Although ultrasensitive detection and analytical technology at the single molecule level is becoming routine, protein extraction techniques have not followed suit and still call for complete lysis that leads to cell death. In principle, with refined extractio...
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ژورنال
عنوان ژورنال: Symmetry
سال: 2020
ISSN: 2073-8994
DOI: 10.3390/sym12030412